The AmScope IN480TC-FL-5MF digital inverted epifluorescence trinocular microscope has interchangeable pairs of 10x22mm and WH25x10mm plan high-eyepoint super-widefield eyepieces, an under-mounted quintuple nosepiece, eight DIN long working-distance infinity plan objectives, fluorescence 100W mercury lamp and Brightfield 30W halogen illumination, a 0.3 NA Kohler condenser with iris diaphragm, and a double-layer mechanical stage with a stage stop to protect slides and objectives from damage. The 5MP fluorescence camera has a CMOS color sensor, image capture and editing software, and USB 2.0 output to capture or display still or video images on a computer or projector. The microscope has a color-corrected infinity optical system (CCIS) that provides color correction and improved focus of the intermediate image across the entire focal plane. The trinocular head has a Siedentopf binocular mount with 50 to 76mm interpupillary adjustment and a fixed 45-degree vertical inclination to reduce eye and neck strain. A Siedentopf binocular head enables the viewer to change the interpupillary distance without changing the tube length, eliminating the need to refocus the image. Anti-mold coatings protect the microscope in high-humidity environments. The microscope includes interchangeable pairs of high-eyepoint super-widefield WH10x22mm plan and WH25x10mm eyepieces. Plan eyepieces provide improved focus over the entire field of view (FOV). High-eyepoint eyepieces ease viewing for viewers who wear glasses. Dioptric adjustment accommodates individual eye-strength differences. The vertical trinocular port accepts a 23mm or C-Mount camera. The under-mounted quintuple nosepiece accepts up to five 20mm objectives at a time. The microscope includes five Brightfield long working-distance infinity plan objectives (4x, 10x, 20x, 40x, and 60x) and three phase-contrast long working-distance infinity plan objectives (10x, 20x, and 40x). Long working-distance plan objectives provide improved focus over the entire range of the viewing field and a longer working distance that is required when viewing specimens in dishes and large containers. Phase-contrast provides high contrast and visibility without the use of stains, allowing specimens to be observed in their natural state without being killed or fixed. An inverted microscope has its light source above the stage pointing downward and the objectives and turret below the stage pointing upward. It is most often used for observing organisms in a large container, such as a well plate or petri dish. It can also be used when specimens require manipulation, or in metallurgical applications. A digital fluorescence microscope is used in biological and metallurgical research and in industrial inspection applications to observe details illuminated by ultraviolet light, and where image capture, detailed records, or documentation is required.
The included AmScope MT5000-CCD 5.0MP (2580 x 1944 pixels) Peltier-cooled fluorescence camera has a 2/3" Sony color CCD sensor for displaying still microscopy images and streaming live videos to a computer monitor or digital TV. The camera can be mounted in any standard C-Mount. The Peltier-cooled sensor provides low-noise fluorescence image acquisition. The sensor has an interline scan mode, an RGB Bayer pattern filter, and a 280mV G-sensitivity. The camera includes image capture and editing software that provides still image and live video capture and editing capability, including measurement functions. Users can select between 2580 x 1944 or 1280 x 932 display resolution. User-defined parameters include brightness, gain, automatic or manual exposure selection, RGB, white balance, and light angle. Exposure duration has a range of 0.1 to 60 minutes. The software supports JPG, TIFF, GIF, PSD, WMF, and BMP file formats and is compatible with Windows XP, Vista, 7, and 8. The camera supports Twain, DirectShow, Amcap, and Minisee file formats. The camera has a USB 2.0 data port (cable included).
The microscope has Brightfield, phase-contrast, and fluorescence illumination. Brightfield and phase-contrast illumination is mounted above the stage and has transmitted 30W halogen illumination. Brightfield (BF) illumination allows the specimen to absorb light, resulting in a dark image on a light background. The phase-contrast element aligns the light source with the phase-contrast objectives to optimize high contrast and visibility without the use of stains. Halogen illumination provides bright light in a concentrated path, and a rheostat controls the amount of light emanating from the lamp. The objectives are mounted on a turret to ease magnification changes. Often used in biological and metallurgical research, fluorescence microscopy uses a xenon or mercury lamp to create ultraviolet light and excitation filters to filter unwanted wavelengths, allowing users to see details undetectable to other light sources. The Kohler condenser has a 0.3 NA to provide a long working-distance of 72mm and 28mm, respectively. Kohler illumination focuses and centers the light path using two iris diaphragms, providing optimum contrast and resolution. The horizon can be rotated 0 to 300 degrees. The double-layer mechanical stage with 1mm stage divisions and 0.1mm vernier resolution locks the slide into place and provides precise slide manipulation along the X- and Y-axes to allow coordinates to be recorded, enabling the viewer to return to a specific location on the slide. The oversize stage is 6.3 x 10 inches (160 x 250mm) (W x D, where W is width, the horizontal distance from left to right; D is depth, the horizontal distance from front to back). It has a traveling range of 4-3/4 x 3-1/8 inches (X- direction x Y-direction). A petri dish holder secures specimens in place. Nested coaxial coarse and fine focus has tension-adjustable coarse focusing and an adjustable lock ring stopper that limits stage range to protect slides and objectives from damage. The focus system has a 38mm working distance, 2mm fine focus, and 0.002mm precision. The total working distance is 6.5mm upward and 2.5mm downward from focus. All-metal mechanical parts, solid-metal frame construction, and a stain-resistant enamel finish provide durability.
|Trinocular port||23mm or C-Mount|
|Eyepieces (30mm)||WH10x22mm plan and WH25x10mm plan|
|Objectives, Brightfield (20mm)||4x, 10x, 20x, 40x, and 60x DIN long working-distance infinity plan objectives|
|Objectives, Phase-contrast (20mm)||10x, 20x, 40x DIN long working-distance infinity plan objectives|
|Stage||Oversize mechanical, with removable stage plate|
|Condenser||Movable 0.3 NA Kohler condenser|
|Light source||Halogen with rheostat, 12V/30W|
|Illumination type||Brightfield, phase-contrast|
|Power||90V to 240V, GS and CE-certified|
|Resolution||5.0MP/2580 x 1944|
|Display type||PC or Mac monitor; Digital TV|
|Camera sensor||2/3" Sony color CCD, model ICX282AQ|
|Exposure duration||0.1 to 60 minutes|
|Frame rate||3 fps at 2580 x 1944 pixels; 10 fps at 1280 x 932|
|Computer connection||USB 2.0|
|File formats||BMP, TIFF, JPG, PICT, PTL|
|Hardware compatibility||Mac and PC|
|Software compatibility||Windows 2000/XP/Vista/7|
|Power source||USB 2.0 or external 3.5V|
Microscopes are instruments used to enhance the resolution of an object or image. Types include compound, stereo, or digital. Compound microscopes use a compound optical system with an objective lens and an eyepiece. Stereo microscopes show object depth in a three-dimensional image. Digital microscopes are used to display an image on a monitor, rather than looking through a lens. Microscopes can have monocular (one), binocular (two), or trinocular (three) eyepieces, with varying magnification abilities. Magnification ability refers to the size of an image. Resolution, also known as resolvant power, refers to the clarity of the image. The interaction between field of view (FOV), numerical aperture (NA), and working distance (WD) determines resolution. Microscopes can control magnification through a fixed focus, or through a range of adjustments. They can also utilize LED, fluorescent, and mirror light sources to help control viewing capabilities. Microscopes are widely used in education, lab research, biology, metallurgy, engineering, chemistry, manufacturing, and in the medical, forensic science, and veterinary industries.
United Scope manufactures microscopy equipment and accessories under the brand name AmScope. The company, founded in 1996, is headquartered in Irvine, CA.