The National Optical 163-PH Trinocular Compound Microscope has three 10x widefield eyepieces (one with pointer), a reverse-mounted nosepiece with four DIN plan phase-contrast objectives that are parcentered and parfocal, halogen illumination, nested coarse and fine focus, a 1.25 NA Abbe condenser with iris diaphragm, and a mechanical stage. The trinocular viewing head has a binocular head with a pair of eyepieces that slide to adjust interpupillary distance, a fixed inclination to reduce eye and neck strain, and 360-degree rotation capability to provide a more comprehensive view and enable sharing; and a vertical tube that has an eyepiece and can be removed to accept a camera adapter (sold separately). An eyepiece pointer in one eyepiece is used to identify features for students. Dioptric adjustment accommodates individual eye-strength differences. A reverse-mounted nosepiece keeps objectives out of the way to prevent damage and contamination, and to enable more secure slide handling. Plan phase-contrast objectives provide enhanced image contrast for viewing live specimens and improved focus over the entire range of the viewing field. Parcentered and parfocal objectives ensure that the image stays centered and focused when the magnification is changed. The 40xR and 100xR objectives are retractable and spring-loaded to prevent damage to the slide or objective when focusing. The 100xR objective is also an oil objective that provides increased resolution over a standard objective. A compound microscope is used for inspection and dissection of specimens when two-dimensional images are desired.
The microscope has lower (diascopic) brightfield illumination that transmits light up through the specimen for enhanced visibility of translucent and transparent objects. Phase-contrast provides high contrast and visibility without the use of stains, allowing specimens to be observed in their natural state without being killed or fixed. The phase turret condenser assembly has one brightfield position. The halogen light source provides bright light in a concentrated path, and a rheostat controls the amount of light emanating from the lamp. A three-position sliding rod can be set to direct light 100% to binocular eyepieces, 100% to vertical tube, or 30% to binocular eyepieces and 70% to vertical tube. The Abbe condenser can be adjusted to control the distance of the light from the stage and has an iris diaphragm to optimize the amount of light illuminating the specimen. The condenser is controlled using a rack-and-pinion mechanism. Blue and green filters are included to help naturalize the light when viewing various samples. The mechanical stage locks the slide into place and provides precise slide manipulation along the X- and Y-axes. Coaxial coarse and fine focus knobs are nested to speed focusing for left-and right-handed viewers, and tension adjustment ensures specimen stays focused during viewing. A stage stop prevents the stage or specimen from coming into contact with the objectives. The cast aluminum frame provides durability and has a gray enamel finish.
|Microscope Head and Optics Specifications|
|Binocular head inclination||30 degrees|
|Interpupillary adjustment||54 to 76mm|
|Eyepieces (18mm)||(2) WF10x, (1) WF10x with pointer|
|Objectives, DIN plan phase-contrast||4x, 10x, 40xR (retractable), 100xR (oil, retractable)|
|Microscope Illumination and Stage Specifications|
|Focus type||Coaxial coarse and fine|
|Condenser||1.25 NA Abbe|
|Light source||Halogen with rheostat, 12V/20W|
|Stage dimensions||140 x 135mm (W x D)|
|Stage travel range||70 x 50mm (X-direction x Y-direction)|
W is width, the horizontal distance from left to right; D is depth, the horizontal distance from front to back.
Microscopes are instruments used to enhance the resolution of an object or image. Types include compound, stereo, or digital. Compound microscopes use a compound optical system with an objective lens and an eyepiece. Stereo microscopes show object depth in a three-dimensional image. Digital microscopes are used to display an image on a monitor, rather than looking through a lens. Microscopes can have monocular (one), binocular (two), or trinocular (three) eyepieces, with varying magnification abilities. Magnification ability refers to the size of an image. Resolution, also known as resolvant power, refers to the clarity of the image. The interaction between field of view (FOV), numerical aperture (NA), and working distance (WD) determines resolution. Microscopes can control magnification through a fixed focus, or through a range of adjustments. They can also utilize LED, fluorescent, and mirror light sources to help control viewing capabilities. Microscopes are widely used in education, lab research, biology, metallurgy, engineering, chemistry, manufacturing, and in the medical, forensic science, and veterinary industries.
National Optical and Scientific Instruments manufactures microscopes and microscope cameras. The company, founded in 1991, is headquartered in Schertz, TX.